An important shortcut to models of disease

FMI News Article
Marc Bühler and his group at the FMI have developed a new and more efficient way to generate embryonic stem cells in which a given gene can be deleted at will, so called conditional knockout cells. They used the engineered nuclease TALEN and a reporter to enrich cells that have integrated defined sequences into the genome through homologous recombination. This refined method makes the use of animals for the generation of conditional knockout cells obsolete. It will also reduce the number of animals required to establish mouse models of disease dramatically. Their new method is therefore in line with 3R efforts around the world.

Link to Press Release

Read the Publication in Cell Reports (Open Access)

Website Bühler Lab