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News›Methods & Protocols on Alternative Splicing

Methods & Protocols on Alternative Splicing

02/08/2022

A new "Methods in Molecular Biology" book was published on "Alternative Splicing: Methods and Protocols". The book was edited by Oriane Mauger and Peter Scheiffele from the University of Basel and features seven articles from NCCR member & associate member labs including one alumni lab.

The following articles were published in the book from NCCR member & associate member labs:

  • Alternative Splicing in Human Biology and Disease - Ruepp lab (NCCR alumni), King’s College London
  • An Optimized Protocol for the Mapping of Cell Type–Specific Ribosome-Associated Transcript Isoforms from Small Mouse Brain Regions - Scheiffele lab, University of Basel
  • Design, Labeling, and Application of Probes for RNA smFISH - Chao lab, FMI Basel
  • Probing the Interactions of Splicing Regulatory Small Molecules and Proteins with U1 snRNP Using NMR Spectroscopy - Allain lab, ETH Zurich
  • Tethered Function Assays to Elucidate the Role of RNA-Binding Proteins - Mühlemann lab, University of Bern
  • Probing Liquid–Liquid Phase Separation of RNA-Binding Proteins In Vitro and In Vivo - Hondele lab, University of Basel
  • Analysis of Oligonucleotide Biodistribution and Metabolization in Experimental Animals - Hall J and Schümperli labs, ETH Zurich

Click here to see the whole table of content of the book.

Website Allain Lab

Website Chao Lab

Website Hall J Lab

Website Hondele Lab

Website Mühlemann Lab

Website Ruepp Lab

Website Scheiffele Lab

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16 August 2022 11:00 – 12:00

Paul Walton - NCCR Equal Opportunity Special Seminar (ZH)

HCI G7, ETH Hönggerberg

22 August 2022 – 26 August 2022All day

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03 October 2022 16:30 – 17:30

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Recent Publications

  • Recursive Editing improves homology-directed repair through retargeting of undesired outcomes
  • Global and precise identification of functional miRNA targets in mESCs by integrative analysis
  • Analysis of Oligonucleotide Biodistribution and Metabolization in Experimental Animals
  • Tethered Function Assays to Elucidate the Role of RNA-Binding Proteins
  • Design, Labeling, and Application of Probes for RNA smFISH

Click here for more information


Recent Preprints

  • Human ATG3 contains a non-canonical LIR motif crucial for its enzymatic activity in autophagy
  • Long-term effects of early postnatal stress on Sertoli cells functions
  • Integrative solution structure of a PTBP1-viral IRES complex reveals strong compaction and ordering with residual conformational flexibility
  • Improved analysis of (e)CLIP data with RCRUNCH yields a compendium of RNA-binding protein binding sites and motifs
  • A novel Smg6 mouse model reveals regulation of circadian period and daily CRY2 accumulation through the nonsense-mediated mRNA decay pathway

Click here for more information


 
Swiss National Science Foundation (SNSF)
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Universität Bern
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ETH Zürich
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École polytechnique fédérale de Lausanne
Friedrich Miescher Institute for Biomedical Research
Universität Basel
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